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Southern blot analysis of pooled DSB-induced recombinants. Genomic DNA from the parental CHO-K1 DRA10 cell line and a pool of neo+ recombinants derived from expression of I-SceI was cleaved with either XhoI–NcoI (X/N) or XhoI–HindIII (X/H). The 5′ neo gene probe (probe A, see Fig. 1) has the same amount of homology to both types of recombination products.
