Figure 3.
Expression of Ca2+-sensing receptor related genes in different tissues of Fugu. Primers were deduced for each of genes such that the fragment included an intron. mRNA preparations were reverse transcribed, and PCR was performed as designed in Materials and Methods. Lane 1 contains genomic DNA; lane 10 is another control in which reverse transcriptase was omitted. The other lanes contain mRNA from the following Fugu organs: 2, brain; 3, nose; 4, stomatic mucosa; 5, muscle; 6, stomach; 7, intestine; 8, kidney; and 9, liver. The marker used was the HinfI-digested pBR322.