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. 1998 Apr 28;95(9):5187–5192. doi: 10.1073/pnas.95.9.5187

Figure 3.

Figure 3

β-Gal activity of cells expressing Wbp1-Cub-PLV together with Nub-fusion proteins. (A) YG0673 cells expressing Wbp1-Cub-PLV and (i) NubI-Alg5, NubA-Alg5, or NubG-Alg5 from a CEN/ARS plasmid; (ii) NubI-Alg5, NubA-Alg5, or NubG-Alg5 from a 2-μm plasmid; (iii) Ost1-NubI, Ost1-NubA, or Ost1-NubG from an integrated fusion gene (no wild-type Ost1p present in the cell); (iv) Ost1-NubI, Ost1-NubA, or Ost1-NubG from a 2-μm plasmid in presence of the wild-type Ost1p. As negative control, YG0673 was transformed with the vector pRS314. Cells were grown on Whatman filters, permeabilized, and incubated in the presence of X-Gal. Expression of β-gal resulted in blue cells. (B) Quantitative β-gal assay of YG0673 cells expressing Wbp1-Cub-PLV together with the vector, low copy number pRS314(NubG-ALG5) (ARS), NubG-ALG5 (2 μm), OST1-NubG (integrated fusion gene, no wild-type OST1 present), and OST1-NubG (2 μm, wild-type OST1 present). Shown are the results of one out of three independent experiments.