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. 1998 Apr 28;95(9):5187–5192. doi: 10.1073/pnas.95.9.5187

Figure 4.

Figure 4

Growth of cells expressing Wbp1-Cub-PLV with various Nub-fusions on agar plates lacking histidine. YG0673 cells expressing Wbp1-Cub-PLV and either NubI-Alg5, NubA-Alg5, or NubG-Alg5 from a 2-μm plasmid or expressing Ost1-NubI, Ost1-NubA, or Ost1-NubG from a 2-μm plasmid were grown to logarithmic phase, spotted in serial 10-fold dilutions on selective agar plates containing 0.2 mM CuSO4 +/− histidine and were incubated for 5 days at 30°C. YG0673 transformed with the vector pRS314 served as negative control.