Figure 5.

Western blot analysis of cells expressing Wbp1-Cub-PLV together with Nub-fusions. YG0673 cells expressing Wbp1-Cub-PLV with the vector (lane 1); with either NubI-Alg5, NubA-Alg5, or NubG-Alg5 from a CEN/ARS plasmid (lanes 2–4); or from a 2-μm plasmid (lanes 5–7). YG0673 expressing Ost1-NubI, Ost1-NubA, or Ost1-NubG as the sole source of Ost1 in the cell (lanes 8–10, integr) or from a 2-μm plasmid in presence of wild-type Ost1p (lanes 11–13). Cells were grown to logarithmic phase in selective medium; proteins were extracted and determined by Western blot analysis and probing with peroxidase-IgG as described in Materials and Methods. ∗, Unspecific degradation product.