Fig. 3. Effect of H2A.Z derivatives on PUR5 and PHO5 transcription. (A) Growth of cells in the absence or presence of 6-AU. Cells were grown and then spotted onto plates containing no 6-AU, 10 µg/ml 6-AU or 25 µg/ml 6-AU. (B) Primer extension analysis of the PUR5 gene in the presence of 6-AU. Induction time in the presence of 6-AU is shown in minutes. The 25S rRNA is shown at the bottom of the figure as a loading control. (C) Transcription of the PUR5 gene in the presence of HU. Primer extension analysis of the PUR5 gene after yeast cells were grown and incubated or not for 3.5 h with 200 mM HU. The 25S rRNA is shown at the bottom of the figure as a loading control. (D) Transcription of the PHO5 gene. Primer extension analyses of the PHO5 locus after yeast cells were grown in the presence (+) or absence (–) of phosphate (Pi). The 25S rRNA is shown at the bottom of the figure as a loading control.