Fig. 5. Extensive, but not limited, movement of silencing is conditioned by SDE1 in transgenic Arabidopsis. (A) Experimental set up. (B) A GFP142 plant transformed with atSUC2-GF-FG. (C) Typical phenotype of GFP142/sde1 plants transformed with atSUC2-GF-FG. (D and E) A leaf of a seedling from the plant in (C) imaged under UV without (D) or with (E) a band-pass filter. (F) Confocal imaging of vein-centred, silenced tissues as seen in (E). G, guard cells. Bar, 300 µm. (G–I) Silencing in a mature leaf of the plant in (C). (H) Acetic acid clearing of the leaf in (G). (I) Overlay of (G) and (H): silencing expands outside the vein network. (J) Transversal section of a mature leaf from a GFP142 plant. Right/middle split channels: GFP and chlorophyll fluorescence, respectively. Left channel: overlay. (K) Transversal section of the stem of a GFP142 plant. Phl, phloem. (L and M) Same as (J) and (K) but in a GFP142/sde1 plant transformed with atSUC2-GF-FG. (N and O) same as (J) and (K) but in a GFP142 plant transformed with atSUC2-GF-FG. v.I, class I vein; v.II, class II vein.