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Inhibition by Bis-ANS of in vitro assembly of C1-82. (A) Kinetics of in vitro assembly of C1-82 (760 pmol) mixed with IRES RNA (38 pmol) in the presence of increasing amounts of Bis-ANS. (B) Electron micrograph of negatively stained NLPs harvested following in vitro assembly. Left panel, control NLPs; Right panel: In vitro assembly in the presence of 60 μM of Bis-ANS. Bar =50 nm.
