Figure 4.

Western blot analysis of SCP-1 expression. Immunoblot analysis with SCP-1 antiserum was performed to measure protein expression levels in cultured glioma cell lines (a) (lanes: 1, T98; 2, Lear; 3, Markus; 4, Cheng; 5, U373; 6, testis) and tissue samples derived from fresh biopsies (b) (lanes: 1 and 3, normal breast samples; 2 and 4, breast cancer tissues; 5, testis). A major band migrating at 110 kDa was detected in lysates of testis, in three glioma cell lines, and in the breast tumor samples. Bands were almost equal in intensity, indicating comparable levels of protein expression. Besides this major band, additional bands were detected in some tumor samples migrating at 120 kDa and 80 kDa.