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. 1998 Apr 28;95(9):5241–5245. doi: 10.1073/pnas.95.9.5241

Figure 1.

Figure 1

Analysis of the reactivity of serum antibodies from CCR5Δ32 donors using confocal laser scanning microscopy and Western blotting. Surface binding of serum from CCR5Δ32 donor 1 (1:50) (A, arrows), murine anti-CCR5 mAb (5 μg/ml) (B, arrows), and serum from a CCR5+/+ individual (1:50) (C) to live CCR5-expressing CHO cells. Similar staining of the cell surface was observed with the CCR5Δ32 sera and the murine anti-CCR5 mAb whereas no surface staining was obtained with CCR5+/+ sera. A subpopulation of CHO cells show no binding of CCR5Δ32 sera or the anti-CCR5 mAb (arrowheads) and neither was staining observed with the two CCR5Δ32 and the four CCR5+/+ sera on nontransfected CHO cells grow n under identical conditions. Western blot analysis (D) of CCR5-expressing CHO cell extract using serum antibodies from CCR5Δ32 donors 1 (lane 1) and 2 (lane 2), and a CCR5+/+ individual (lane 3) or nontransfected CHO cells using serum antibodies from CCR5Δ32 donor 1 (lane 4).