Figure 3.
(a) Immunoblots of PL from crude membranes of left ventricles from control uninfected rats or rats 2 and 7 days after infection with 5 × 109 pfu of either Ad.βgal, Ad.EGFP, or Ad.RSV.PL. (b) Protein levels of PL in preparations from uninfected hearts (n = 8), preparations of hearts infected with Ad.βgal at day 2 (n = 6), preparations of hearts infected with Ad.EGFP at day 2 (n = 4), preparations of hearts infected with Ad.PL at day 2 (n = 8), preparations of hearts infected with Ad.βgal at day 7 (n = 6), preparations of hearts infected with Ad.EGFP at day 7 (n = 4), and preparations of hearts infected with Ad.PL at day 7 (n = 8). ∗, P < 0.05 compared with uninfected, Ad.βgal at 2 days, and Ad.EGFP at 2 days. #, P < 0.05 compared with Ad.βgal at 2 days, and Ad.EGFP at 7 days. There were no significant differences between the PL protein levels in the uninfected group and Ad.βgal or Ad.EGFP (P > 0.2). (c) Immunoblots with mAbs to the of ryanodine receptor, Na/Ca exchanger, SERCA2a, and calsequestrin from crude membrane of left ventricles after 2 days of infection with 5 × 109 pfu of either Ad.βgal, Ad.EGFP, or Ad.RSV.PL.