Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1975 Sep;32(3):373–379. doi: 10.1038/bjc.1975.237

Immunoreactive calcitonin production by human lung carcinoma cells in culture.

M Ellison, D Woodhouse, C Hillyard, M Dowsett, R C Coombes, E D Gilby, P B Greenberg, A M Neville
PMCID: PMC2024744  PMID: 1233082

Abstract

Monolayer cultures have been established from a poorly differentiated carcinoma of the lung. Homogeneous cell growth and morphology have been maintained for over 18 months through more than 80 subculture passages, and the cells have been found to produce both immunoreactive calcitonin and an immunoreactive carcinoembryonic antigen-like material.

Full text

PDF
373

Images in this article

375Fig. 1
on p.375

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Addison G. M., Hales C. N., Woodhead J. S., O'Riordan J. L. Immunoradiometric assay of parathyroid hormone. J Endocrinol. 1971 Mar;49(3):521–530. doi: 10.1677/joe.0.0490521. [DOI] [PubMed] [Google Scholar]
  2. Braunstein H., Stephens C. L., Gibson R. L. Secretory granules in medullary carcinoma of the thyroid. Electron microscopic demonstration. Arch Pathol. 1968 Mar;85(3):306–313. [PubMed] [Google Scholar]
  3. Breborowicz J., Easty G. C., Birbeck M., Robertson D., Nery R., Neville A. M. The monolayer and organ culture of human colorectal carcinomata and the associated "normal" colonic mucosa and their production of carcinoembryonic antigens. Br J Cancer. 1975 May;31(5):559–569. doi: 10.1038/bjc.1975.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark M. B., Boyd G. W., Byfield P. G., Foster G. V. A radioimmunoassay for human calcitonin M. Lancet. 1969 Jul 12;2(7611):74–77. doi: 10.1016/s0140-6736(69)92389-7. [DOI] [PubMed] [Google Scholar]
  5. Coombes R. C., Hillyard C., Greenberg P. B., MacIntyre I. Plasma-immunoreactive-calcitonin in patients with non-thyroid tumours. Lancet. 1974 Jun 1;1(7866):1080–1083. doi: 10.1016/s0140-6736(74)90557-1. [DOI] [PubMed] [Google Scholar]
  6. Deftos L. J., Roos B. A., Bronzert D., Parthemore J. G. Immunochemical heterogeneity of calcitonin in plasma. J Clin Endocrinol Metab. 1975 Mar;40(3):409–412. doi: 10.1210/jcem-40-3-409. [DOI] [PubMed] [Google Scholar]
  7. Kalina M., Pearse A. G. Ultrastructural localization of calcitonin in C-cells of dog thyroid; an immunocytochemical study. Histochemie. 1971;26(1):1–8. doi: 10.1007/BF00307777. [DOI] [PubMed] [Google Scholar]
  8. Laurence D. J., Stevens U., Bettelheim R., Darcy D., Leese C., Turberville C., Alexander P., Johns E. W., Neville A. M. Role of plasma carcinoembryonic antigen in diagnosis of gastrointestinal, mammary, and bronchial carcinoma. Br Med J. 1972 Sep 9;3(5827):605–609. doi: 10.1136/bmj.3.5827.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mackay A. M., Patel S., Carter S., Stevens U., Laurence D. J., Cooper E. H., Neville A. M. Role of serial plasma C.E.A. assays in detection of recurrent and metastatic colorectal carcinomas. Br Med J. 1974 Nov 16;4(5941):382–385. doi: 10.1136/bmj.4.5941.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Milhaud G., Calmette C., Taboulet J., Julienne A., Moukhtar M. S. Letter: Hypersecretion of calcitonin in neoplastic conditions. Lancet. 1974 Mar 16;1(7855):462–463. doi: 10.1016/s0140-6736(74)92428-3. [DOI] [PubMed] [Google Scholar]
  11. Pearse A. G., Polak J. M. Endocrine tumours of neural crest origin: neurolophomas, apudomas and the APUD concept. Med Biol. 1974 Feb;52(1):3–18. [PubMed] [Google Scholar]
  12. Powles T. J., Clark S. A., Easty D. M., Easty G. C., Neville A. M. The inhibition by aspirin and indomethacin of osteolytic tumor deposits and hypercalcaemia in rats with Walker tumour, and its possible application to human breast cancer. Br J Cancer. 1973 Oct;28(4):316–321. doi: 10.1038/bjc.1973.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rees L. H., Ratcliffe J. G. Ectopic hormone production by non-endocrine tumours. Clin Endocrinol (Oxf) 1974 Jul;3(3):263–299. doi: 10.1111/j.1365-2265.1974.tb01801.x. [DOI] [PubMed] [Google Scholar]
  14. Reynolds J. J. Inhibition by calcitonin of bone resorption induced in vitro by vitamin A. Proc R Soc Lond B Biol Sci. 1968 May 14;170(1018):61–69. doi: 10.1098/rspb.1968.0024. [DOI] [PubMed] [Google Scholar]
  15. Silva O. L., Becker K. L., Primack A., Doppman J., Snider R. H. Ectopic secretion of calcitonin by oat-cell carcinoma. N Engl J Med. 1974 May 16;290(20):1122–1124. doi: 10.1056/NEJM197405162902007. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES