Figure 6.

Representative Southern blots probed with full-length FcγRIII (A), FcɛRII (B), and 157-bp RPL7 human cDNA probes. Paired human ASM samples were incubated with control (CO) or atopic asthmatic (SE) serum for 24 hr. cDNA was transcribed from total RNA primed with oligo(dT). PCR products were amplified by using human-specific FcγRIII, FcɛRII, and RPL7 primers, and then probed. Note the marked induction of FcɛRII expression in the atopic asthmatic serum-sensitized sample (i.e., >7.5-fold), whereas expression of FcγRIII or RPL7 was similar in both samples.