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. 1998 Apr 28;95(9):5263–5268. doi: 10.1073/pnas.95.9.5263

Figure 1.

Figure 1

Inhibition of ⋅OH generation by desferri-exochelins. Production of ⋅OH was quantitated by measurements of the hydroxylated salicylate isomers 2,3-DHBA and 2,5-DHBA by GC/MS. A ⋅OH generating system was created by adding 200 μM of salicylic acid to 200 μM xanthine sodium (X), 4.4 milliunits/ml of purified xanthine oxidase (XO), and iron supplied as 1 μM FeNTA. D-EXO is desferri-exochelin 772SM, and Fe-EXO is iron-saturated exochelin 772SM. Duplicate measurements are shown. When X, XO, and FeNTA were present, 2,3- and 2,5-DHBA were produced in the absence of iron chelators or in the presence of Fe-EXO. However, in the presence of 10 μM deferoxamine mesylate or 4 μM D-EXO, production of 2,3- and 2,5-DHBA was suppressed (by preplanned ANOVA, P < 0.01 for 2,3-DHBA and P < 0.001 for 2,5-DHBA). Therefore, both deferoxamine and a desferri-exochelin prevented generation of ⋅OH by iron chelation in this cell-free system.