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. 1998 Apr 28;95(9):5275–5280. doi: 10.1073/pnas.95.9.5275

Figure 1.

Figure 1

Strategy for inactivation of the murine GstP gene cluster. (A) GstP1/P2 gene cluster and (B) targeted GstP1/P2 allele. Restriction endonucleases: B, BglII; E, EcoRI; Ev, EcoRV; K, KpnI; N, NheI; P, PstI. A cassette containing the en-2A splice acceptor site, internal ribosome binding site element, lacZ, neo (βGeo), and simian virus 40 polyadenylation site, replaces genomic DNA from the EcoRI site in GstP2, exon 5, to the first PstI site in the 3′ untranslated region of GstP1. Coding exons are represented by black boxes; white boxes represent the region of DNA used as 5′- and 3′-flanking probes for Southern screening. (C) Southern blot analysis of ES cell genomic DNA from targeted clones. The 19-kb KpnI fragment represents the wild-type allele, and the 11.5-kb band corresponds to the targeted allele, using the 5′-flanking probe. (D) Southern blot analysis of tail-tip genomic DNA from wild-type (+/+), heterozygous (+/−), and null (−/−) mice. The 7-kb EcoRI fragment represents the wild-type allele, whereas the 6.3-kb band corresponds to the targeted allele, using the 3′-flanking probe.