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. 1998 Apr 28;95(9):5275–5280. doi: 10.1073/pnas.95.9.5275

Figure 2.

Figure 2

Characterization of GstP1/P2(−/−), GstP1/P2(+/−), and GstP1/P2(+/+) mice. Samples were from male (M) and female (F) wild-type (+/+), heterozygote (+/−), and null (−/−) mice. (A) Northern analysis of hepatic RNA (10 μg per lane), using a cDNA representing full-length GstP1. Sample integrity was confirmed by staining of 16S and 28S rRNA in the gel with ethidium bromide before transfer to nitrocellulose, and equivalence of loading was confirmed by using a cDNA for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (B) Western blot analysis of hepatic cytosol (10 μg protein per lane), using a polyclonal antiserum raised against the mouse GstP1–2 protein and ECL detection (Amersham). Equivalence of loading was confirmed by Coomassie blue staining of a duplicate SDS/PAGE gel. (C) Enzymatic activity of hepatic cytosol toward ethacrynic acid and CDNB was measured spectrophotometrically. Data represent mean of five animals ± SEM. (D) Western blot analysis of hepatic cytosol (10 μg protein per lane), using antisera toward GST from alpha and mu families (38, 39), and ECL detection (Amersham). Equivalence of loading was confirmed by Coomassie blue staining of a duplicate SDS/PAGE gel.