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Expression of GSH-dependent enzymes in GstP null mice. Samples were from male (M) and female (F) wild-type (+/+), heterozygote (+/−), and null (−/−) mice. Western blot analysis of hepatic cytosolic samples (10 μg protein per lane), with antisera to GSH synthetase and γ-glutamylcysteine synthetase (light chain). Total GSH content was determined in hepatic cytosol as detailed in Materials and Methods. Values are expressed as mean ± SEM from five determinations.
