Figure 6.

p300 activates HIV_bru Vpr⁺ replication. (A) Time course for p300 activation of HIV replication stimulated by p300. 293 cells expressing the CD4 molecule were transfected with 6 μg of CMV-p300 or a control CMV plasmid with no insert. Cells were challenged with HIV 24 h later, and replication was measured by RT assays at 0, 2, and 4 days after infection. RT activity is expressed as mean values ± standard deviations of triplicate assays. (B) p300 differentially regulates HIV_bru Vpr⁻ and HIV_bru Vpr⁺ replication. UM-316 cells were transfected with 0.5 μg of CMV-CD4 and either 5 μg of a control CMV vector or CMV-p300, as indicated. Cells were infected with HIV_bru Vpr⁻ or HIV_bru Vpr⁺ 24 h later, and viral replication was measured on day 4 by RT assays. RT activity is expressed as mean values ± standard deviations of triplicate assays.