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Gel mobility shift analysis of the binding of Ros and Ros mutant proteins to the promoters of virC and virD operons. Lanes: 1–3, wild-type Ros protein and C82A and H92A mutant Ros proteins, respectively; 4, target DNA fragment only. A 210-bp PvirC/virD fragment labeled with [α-32P]dATP was used as a probe.
