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. 1998 Apr 28;95(9):5299–5304. doi: 10.1073/pnas.95.9.5299

Figure 1.

Figure 1

Plasmid maps for Hly secretion by r-BCG strains. (A) Extrachromosomal Hly expression by E. coli–mycobacteria shuttle plasmid pAT261:Hly. Insertion of the pILH-1-derived 1.7-kb PstI fragment encoding the DNA sequence of the mature Hly protein. Mrep, mycobacterial replicon; Erep, E. coli origin of replication; kan, kanamycin-resistance gene; hsp, heat shock protein promoter. (B) Chromosomal integrative shuttle vector pMV306:Hly for Hly expression by mycobacteria. The inserted DNA restriction fragment (XbaI–SalI) including the hsp60 promoter is derived from plasmid pAT261:Hly. attP, attachment site of mycobacteriophage L5; MCS, multiple cloning site; int, integrase of mycobacteriophage L5.