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Analysis of Hly expression by r-BCG. Detection of Hly fusion protein in lysates (lanes L) or supernatants (lanes S) of BCG, BCG pAT261:Hly, or BCG pMV306:Hly strains by immunostaining. The primary antibody used for chemiluminescent immunostaining of the 62-kDa Hly hybrid protein was anti-Hly mAb H14–3.
