Table 1.

TP53 HRM primers and PCR annealing temperature conditions

Exon	Primer name	Sequence	Annealing conditions
5a	TP53_Exon5a_F	CAACTCTGTCTCCTTCCTCTTCCTAC	65–60°C touchdown 0.5°C/cycle for 10 cycles
	TP53_Exon5a_R	AGCCATGGCACGGACGCG
5b	TP53_Exon5b_F	CTCCTGCCCGGCACCCGC	65–60°C touchdown 0.5°C/cycle for 10 cycles
	TP53_Exon5b_R	CTAAGAGCAATCAGTGAGGAATCAGA
6	TP53_Exon6_F	CAACCACCCTTAACCCCTCCT	68–58°C touchdown 1.0°C/cycle for 10 cycles
	TP53_Exon6_R	AGACGACAGGGCTGGTTGC
7	TP53_Exon7_F	AGGCGCACTGGCCTCATC	68–58°C touchdown 1.0°C/cycle for 10 cycles
	TP53_Exon7_R	GAGGCTGGGGCACAGCA
8	TP53_Exon8_F	GACCTGATTTCCTTACTGCCTCTTG	63.5–58.5°C touchdown 0.5°C/cycle for 10 cycles
	TP53_Exon8_R	AATCTGAGGCATAACTGCACCCTT

Underlined nucleotides are introduced sequence