Table 2.

TP53 sequencing primers and annealing temperature conditions

Exon	Primer name	Sequence	Annealing temperature
5	TP53_5F	CTCTGTCTCCTTCCTCTTCC	55°C
	TP53_5R	GCAATCAGTGAGGAATCAGAGG
6	TP53_6F	AGATAGCGATGGTGAGCAGC	60°C
	TP53_6R	ACTGACAACCACCCTTAACC
7	TP53_7F	CAGGTCTCCCCAAGGCGCAC	60°C
	TP53_7R	GCAAGCAGAGGCTGGGGCAC
8	TP53_8F	GGAGTAGATGGAGCCTGGTT	58°C
	TP53_8R	GTGAATCTGAGGCATAACTG