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. 2007 Jul 13;93(9):3120–3131. doi: 10.1529/biophysj.107.104737

FIGURE 3.

FIGURE 3

Deposition of Atto 647-WGA onto a coated glass slide. (A) Fluorescence image of molecules delivered by one pulse (−10 V, 300 ms) from the pipette. The scale bar is 5 μm. (B) Line scan through the fluorescent spot with Gaussian fit, showing a feature size of 0.9 μm full width at half-maximum. (C) Intensity versus time traces from the low-intensity spots surrounding the bright feature in A, showing single- and double-step photobleaching. (D) Intensity versus time trace obtained by confocal illumination and detection at the pipette tip under application of voltage pulses (−10 V, 300 ms). The beginning of the pulses are marked by arrows. The inset shows a region with high time resolution so that bursts caused by individual molecules are visible.