FIGURE 5.

(A) Image of the head of the sperm cell attached to the coverslip under white light illumination. (B) Fluorescence image showing a single Atto 647-labeled WGA molecule (arrow) on the cell membrane. Shown is one frame of a video (available as Supplementary Material). (C) Overlay of the white light and fluorescence images. Scale bars are 5 μm. (D) Typical trajectory on the anterior acrosomal region obtained by tracking a single WGA molecule, the square and triangle show the start and end of the trajectory, respectively. The idealized outline of a typical boar sperm cell is also shown. (E) Typical trajectory on the postacrosomal region.