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. 2007 Jul 13;93(9):3120–3131. doi: 10.1529/biophysj.107.104737

FIGURE 6.

FIGURE 6

Analysis of trajectories. (A) MSD versus time lag for the WGA molecules diffusing on the acrosome and postacrosome. For a given time lag the average of the MSD of 65 individual trajectories for the acrosome and 37 trajectories for the postacrosome region is shown. The data points and error bars give the mean ± SE. The diffusion coefficients were determined from the slope of linear fits through data points 2–10 (shown in the graph). (B and C) Histogram of diffusion coefficients of individual trajectories in the (B) acrosomal region, and (C) postacrosomal region of the sperm cell. A Gaussian fit to the histogram (continues gray curve) was added as a guide to the eye. A theoretical probability distribution (black dash dot curve) for trajectories with 84 data points is shown for comparison. This has been multiplied with the number of events and the bin width and divided by 3 for presentational purposes.