FIGURE 9.

Schematic showing how diffusional boundaries were probed using the measured trajectories. A six pixel wide boundary region 3 (black line) was inserted between region 1 (white) and region 2 (gray). When a molecule moves into the boundary region, this was counted as an attempted crossing event. When the molecule moves from region 1 to region 2 (or vice versa) this was counted as a successful crossing event. The crossing probability was calculated by dividing successful crossing events by attempted crossings.