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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Cell Signal. 2007 Mar 30;19(8):1733–1744. doi: 10.1016/j.cellsig.2007.03.010

Figure 7.

Figure 7

Role of tyrosine phosphorylation of GIT1 on paxillin binding.

The indicated GIT1/Flag tyrosine-to-phenylalanine mutants were transfected into COS7 cells together with chicken paxillin. Cells were scraped and lysed after 48 hours, and soluble lysates immunoprecipitated by addition of M2 Flag-agarose beads. The 3YF mutant is Y293F/Y392F/Y607F. Co-immunoprecipitated paxillin and endogenous β–PIX were measured by immunoblotting with specific antisera, and precipitated Flag-GIT1 directly visualized with M2 Flag-HRP conjugate.