Figure 2.

Analysis of recombinant lines transfected with pTEXTcTRtdm. (A) Structure of expression plasmid. The tryA coding region, the bacterial neomycin phosphotransferase (neo) gene, and GAPDH untranslated regulatory sequences are represented by open, grey, and filled boxes, respectively. The location of polyadenylation (A⁺) and splice acceptor sites (SAS) within regulatory regions is indicated; also shown is the unique EcoRI restriction site. (B) DNA blot analysis. Total DNA isolated from indicated lines was digested with EcoRI, size-fractionated, and blotted onto nylon; the membrane was sequentially probed with radiolabeled neo and tryA sequences. Sizes of molecular markers are given in kilobases.