Figure 2.

Primary structure of a portion of the CR protein derived from ABRM of M. galloprovincialis. Areas shown by the double underlines were used as primer regions for obtaining the cDNA sequence from which the amino acid sequence was derived. Also shown are the homologous regions of twitchin from A. californica (27) and C. elegans (29). The primer region of Mytilus twitchin as well as 8-Leu to 19-Val were determined by N-terminal amino acid sequencing, and the latter sequence was confirmed by the cDNA. The numbers represent residues from the N-terminal end of the fragment or molecule. The underlined peptide was synthesized and used to obtain a polyclonal antibody to the protein. Identical and gapped amino acids are shown by dots and dashes, respectively, for the best alignment of the three sequences.