Figure 2.
Localization of the region of CTR1 that associates with ETR1. (A) Results of the yeast two-hybrid assay. β-Galactosidase activity [in modified Miller units (22)] was measured in yeast transformants expressing DB–ETR1293–729 plus each of the indicated AD–CTR1 fusion proteins. Fusions 1 and 2 were expressed in plasmid pGAD424; fusions 3–7 were expressed in plasmid pACTII (which has a stronger promoter than pGAD424). Three transformants of each were tested, and the average ± the SD is presented. The background level of activity for transformants carrying the pACTII vector is given. (B) Immunoblot analysis of the levels of AD fusion proteins in the transformants using antibody to Gal4 (AD). Two of each of the transformants tested for β-galactosidase activity were examined and were found to be similar; a representative of each is shown. The exposure for construct 1 was approximately four times longer than for the other constructs.