(A) Southern blot analysis of aspen genomic DNA (10 μg per lane) digested with BamHI (lane B) and XbaI (lane X), probed with 32P-labeled Pt4CL1 and Pt4CL2 cDNAs, respectively. λ DNA digested with HindIII was used as size markers, and values are given in kb. (B) Northern blot analysis of total RNA (10 μg per lane) from young leaf (lane L), top internodes (TI), lower internodes (LI), and secondary developing xylem (X) of aspen, probed with 32P-labeled Pt4CL1 and Pt4CL2 cDNAs, respectively.