Histochemical analysis of GUS gene expression in transgenic tobacco. Transverse sections of the stem (A, bar = 100 μm) and leaf (B, bar = 100 μm) excised from Pt4CL1p-GUS transgenic tobacco, and transverse sections of the stem (C, bar = 250 μm) and leaf (D, bar = 100 μm) from Pt4CL2p-GUS transgenic tobacco were stained for GUS activity as described (17).