Table 2.

Structures of most potent ACE inhibitors identified from individual bead assay and activity of purified compounds

Compound no.	Mean inhibition from single beads, % (n)	IC50 for purified compounds, nM
		Mixture of isomers	Separated Diastereomers
			l-Pyrrolidine	d-Pyrrolidine
1	91  ± 8 (5)	0.61	0.16	>100
2	88  ± 5 (4)	1.3
3	82  ± 5 (2)	13
4	81  ± 13 (3)	4.0	0.64	>100
5	84  ± 8 (3)
6	81 (1)
7	76  ± 2 (3)	32
8	69  ± 4 (2)	400

Note that the stereochemistry of the pyrrolidine ring is shown in the l-configuration for convenience.