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Susceptibility of natively supercoiled recombination substrates to S1 nuclease digestion. L, 1-kb ladder fragments; N, native plasmid; oc, open-circular; lin, linear; sc, supercoiled plasmid. The graph shows the ratio of supercoiled plasmid remaining after 30 min of S1 digestion at the specified temperature to the amount present before the addition of S1 nuclease.
