Figure 5.
Recombination-product distributions at reduced superhelix density (σavg = −0.030 ± 0.005). (A) Control plasmids. (B) A-tract-containing plasmids. Reduced superhelix-density substrates were prepared by relaxing natively supercoiled plasmids with topoisomerase I in the presence of ethidium bromide. Superhelix densities were determined by one-dimensional agarose gel electrophoresis in the presence of chloroquine. Recombination reaction conditions and figure annotations are identical to those in Fig. 2.