Figure 2.
Interaction of thermally inactivated AcChoEase with DMPC liposomes as revealed by gel filtration. Samples of Torpedo AcChoEase (4 × 10−7 M) in buffer 1 were incubated at 34°C in the presence of DMPC liposomes (5 mg/ml). After 10 min, when AcChoEase activity was <0.5%, the reaction mixture was applied to a Sephacryl S-300 column; protein and lipid in the effluent were monitored by measuring, respectively, fluorescence and absorbance at 340 nm. (•), Fluorescence at 340 nm; (○), OD at 340 nm. Arrow marks the position of thermally inactivated AcChoEase in the absence of liposomes.