Figure 5.

Immunohistochemical analysis of ACS4. Tissue sections from rat adrenal gland (A and B), ovary (C and D), and testis (E) were analyzed with rabbit anti-ACS4 IgG. In A and B, the intensity of immunostaining in adrenal gland is highest in the zona fasciculata (F) and reticularis (R), relatively weak in the zona glomerulosa (G), and not detected in the capsule or medulla (M). In C, note significant immunoreactivity in the large cells (indicated by **), but not in the small cells (indicated by ∗) of the corpus luteum. Immunoreactivity was not undetected in theca or granulosa cells of the growing follicle (F), theca cells of atretic follicle (A), or vascular endothelial cells (indicated by arrows). In D, immunoreactivity is present in stromal luteinized cells (arrows). In E, Immunoreactivity is present in Leydig cells (L) but not in the cells of the seminiferous tubules (S). (Bars = A–D, 200 μm; E, 100 μm.)