Table 1.

Summary of the purification of recombinant ACS4

Step	Total protein, mg	Total activity, μmol/min	Specific activity, μmol per min per mg	Yield, %
Crude extract	1360	883	0.65	100
Triton X-100 extract	1040	698	0.67	79
Red-Toyopearl	144	630	4.37	71
Blue-Toyopearl	8.4	178	21.1	20

A 500-ml culture of E. coli cells carrying pTV-ACS4 was used to prepare the crude extract. Enzyme activity was measured by the isotopic method with palmitate as a substrate.