Figure 3.

Luc⁺⁺ luminescence elicited by anaerobically grown E. coli. Overnight aerobic cultures in LB medium were diluted 1000-fold into fresh LB plus 0.25% glucose and further incubated for 4.5 hr in gas pack jars. The jars were opened, the cultures were shaken in air at 200 rpm for 2 min, 0.78-ml aliquots were mixed with 20 μl of 2.0 mM Luc⁺⁺, and luminescence was averaged from three consecutive 10-sec integrate periods separated by 5-sec delays. Bars: 1, AB1157; 2, sodA; 3, sodB; 4, sodA sodB.