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Immunocytochemical localization of AT-1 protein. The stably transfected cells, HeLa/GT3+/AT-1 (A) and HeLa/GT3+/pcDNAI (B), were fixed, permeabilized with saponin, and incubated with affinity-purified rabbit antibody specific to AT-1 protein, followed by incubation with fluorescein isothiocyanate-conjugated anti-rabbit IgG (Jackson ImmunoResearch). (Bar = 20 μm.)
