Figure 5.

Time-dependent incorporation of radioactivity into semi-intact cells from [Ac-¹⁴C]Ac-CoA (A) and the effect of CoA on the incorporation (B). (A) Trypsinized cells were permeabilized by treatment with SLO and incubated with [Ac-¹⁴C]Ac-CoA [35 μM, 56 mCi/mmol (1 Ci = 37 GBq); Amersham]. The incorporated radioactivity was determined as described in the text. The open circles represent HeLa/GT3⁺/pcDNAI and the filled circles HeLa/GT3⁺/AT-1. (B) Indicated amounts (70 and 350 μM) of CoA were added in the reaction mixtures. After 4-hr incubation, the incorporated radioactivities were measured as described. Values are shown as a ratio relative to the control experiment in the absence of CoA using SLO treated HeLa/GT3⁺/AT-1 cells. The results represent the mean and standard error derived from three replicate assays.