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. 1997 Apr 1;94(7):2909–2914. doi: 10.1073/pnas.94.7.2909

Figure 1.

Figure 1

In vitro mixed RNA synthesis using lacUV5 promoter and f1 minus-strand origin. (A) Electropherogram of RNA products synthesized in the standard reaction that contained 0.1 pmol of template DNA and 0–0.8 pmol of competiter DNA as indicated. (Left) lacUV5–2 as template and lacUV5–1 as competitor. (Center) f1 ssDNA as template and lacUV5–1 as competitor. (Right) lacUV5–1 as template and f1 ssDNA as competitor. Molar ratio of template to competitor is shown above each lane. Position of each RNA product is indicated on the right. (B) Quantification of the data shown in A. Amounts of RNA products were measured by densitometry of autoradiographs using Fuji BAS 2000 system. ○, f1 ssDNA as template and lacUV5–1 as competitor; □, lacUV5–1 as template and f1 ssDNA as competitor; ▪, lacUV5–2 as template and lacUV5–1 as competitor; •, theoretical curve for a template and a competitor of identical affinity.