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Effect of SSB on the size of in vitro-amplified DNA. Amplification assays were carried out as described, using 0.5 ng of φ29 TP-DNA as input template. Lanes 1–6 contained 6, 2, 0.7, 0.2, 0.07, and 0.02 μg of SSB, respectively. After 1 hr of incubation at 30°C, reactions were stopped and the size of the amplified DNA was analyzed by alkaline agarose gel electrophoresis followed by autoradiography. The electrophoretic mobility of full-length φ29 DNA and short DNAs is indicated.
