Figure 4.

Nature of the in vitro-amplified short DNAs. (Upper) HindIII restriction map of φ29 DNA. The fragments corresponding to the DNA ends are highlighted and their size is indicated. (Lower) Southern blot analysis of short DNAs. Amplification assay was carried out as described, using 0.5 ng of φ29 TP-DNA as input template, in the absence of SSB, so only short DNAs were amplified. This sample was blotted against HindIII-digested φ29 DNA (S), as described. The position of the fragments corresponding to the φ29 DNA ends is indicated in the control lane (C), in which internally labeled full-length φ29 DNA was used as probe.