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Alignment of ch-uPA sequence with the putative PAI binding site of h-uPA. ch-uPA was aligned with Pro-171 in h-uPA (32). The positively charged amino acids of the putative PAI binding site in h-uPA (20) are underlined. The ch-uPA amino acid residues targeted for substitution are in boldface type. In the comparison of chicken and human uPA sequences, identical amino acids are indicated by a vertical line and conservative substitutions are indicated by a diamond.
