Figure 2.
Activation of chicken plasminogen by ch-uPAwt and ch-uPARRHR, and sensitivity of ch-uPAwt and ch-uPARRHR to benzamidine. (A) ch-uPAwt (▵, 25 pg; ○, 50 pg) or ch-uPARRHR (▴, 25 pg; •, 50 pg) was assayed for plasminogen activation in a coupled chromogenic substrate assay where the cleaved product is monitored at A405 (22). (B) Inhibition of chicken mutant uPA (ch-uPARRHR) and chicken wt uPA (ch-uPAwt) by benzamidine. ch-uPARRHR (•, 50 pg), or ch-uPAwt (○, 50 pg) was incubated with varying concentrations of benzamidine and assayed for activity in a chromogenic substrate assay. The activity of ch-uPA in the absence of added benzamidine was assigned a value of 100%.