Figure 3.
The effect of PAI-1 on h-uPA, ch-uPAwt, and ch-uPARRHR catalytic activity and electrophoretic mobility. ch-uPARRHR (A and B, 50 pg), or ch-uPAwt (A and C, 50 pg) was incubated either without (B and C, lane 1), or with human rPAI-1 in the various molar amounts indicated and analyzed for residual uPA activity (A) and electrophoretic mobility by casein-plasminogen underlay (B and C). The underlay was incubated at 37°C for 3 days. The positions of prestained molecular weight markers are given in kDa on the left. The arrowhead indicates the position of the uPA–PAI-1 complexes.