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. 2001 Jul 15;108(2):223–232. doi: 10.1172/JCI10716

Figure 2.

Figure 2

PDC-E2 is not cleaved during apoptosis. Reduced NRC lysates from control cells (C) and apoptotic (A) cells were immunoblotted with three different PBC patient sera (lanes 1–6) and SLE patient sera monospecific for NuMA (lanes 7 and 8, top panel), PARP (lanes 7 and 8, middle panel), and U1 70K (lanes 7 and 8, bottom panel). Apoptosis was induced by UV-B irradiation. Equal amounts of protein were electrophoresed in each gel lane. Neither loss of intact PDC-E2 (66 kDa) nor any PDC-E2 cleavage fragments were seen in the apoptotic lysates (lanes 2, 4, and 6) compared with control lysates (lanes 1, 3, and 5, respectively). Likewise, no cleavage of any other PBC autoantigen was detected. Blotting of NuMA, PARP, and U1 70K in the apoptotic lysate showed generation of their expected caspase cleavage fragments (lane 8), confirming induction of apoptosis. Molecular size markers (Mr × 10–3) are shown on the left. Blotting by each serum was examined at least three times with identical results. A representative blot is shown.