Figure 2.

Northern blot analysis of RET-RGS-d expression in various bovine tissues. Twenty micrograms of total RNA prepared from various bovine tissues (see Materials and Methods) was analyzed in each lane. The blot was serially hybridized with an RET-RGS d-probe (A) and then, as a control for RNA loading and integrity, with a human β-actin probe (B). Autoradiographs were exposed 40 h for A and 20 h for B at −70°C with intensifying screens.